The density of KAHRP-labelled ring structures increases during parasite development.

(A) CS2-infected RBC membranes imaged by dSTORM and SEM during knob formation. Representative 1 x 1 μm sections are presented from cells at 16, 20, 24 and 30 h post-invasion (hpi). Scale bar: 500 nm. (B) The numbers of puncta (structures 5–50 pixels in size) and ring structures were quantified for multiple regions from different cells (n = 35, 25, 26 and 15 membranes for 16, 20, 24 and 30 h post-invasion respectively). Data is presented as the mean ± SEM (unpaired t-test, * p < 0.05, ** p < 0.005, *** p < 0.0001).