A 4-oxalocrotonate tautomerase (4-OT) trimer has been isolated from Burkholderia lata, and a kinetic, mechanistic, and structural analysis has been performed. The enzyme is the third described oligomer state for 4-OT along with a homo- and heterohexamer. The 4-OT trimer is part of a small subset of sequences (133 sequences) within the 4-OT subgroup of the tautomerase superfamily (TSF). The TSF has two distinct features: members are composed of a single β–α–β unit (homo- and heterohexamer) or two consecutively joined β–α–β units (trimer) and generally have a catalytic amino-terminal proline. The enzyme, designated as fused 4-OT, functions as a 4-OT where the active site groups (Pro-1, Arg-39, Arg-76, Phe-115, Arg-127) mirror those in the can...
The genes (caaD1 and caaD2) encoding the trans-3-chloroacrylic acid dehalogenase (CaaD) of the 1,3-d...
The tautomerization of 2-oxo-4E-hexendioate by 4-oxalocrotonate tautomerase has been studied by quan...
Tautomerase superfamily members have an amino-terminal proline and a beta-alpha-beta fold, and inclu...
Biocatalysis is a branch of biochemistry that exploits the ability of an enzyme to convert a substra...
The tautomerase superfamily (TSF) is a group of relatively short monomers (60-150 amino acid residue...
4-Oxalocrotonate tautomerase (4-OT) and trans-3-chloroacrylic acid dehalogenase (CaaD) are members o...
4-Oxalocrotonate tautomerase (4-OT) and trans-3-chloroacrylic acid dehalogenase (CaaD) are members o...
The enzyme 4-oxalocrotonate tautomerase (4-OT) is part of a catabolic pathway for aromatic hydrocarb...
The enzyme 4-oxalocrotonate tautomerase (4-OT) is part of a catabolic pathway for aromatic hydrocarb...
Enzymes are categorized into families based on sequence similarity and conserved features. Superfami...
textP[Kappa]a is not responsible for the improved activity. Hence, stabilization of an enediolate in...
Gene duplication and fusion are among the primary natural processes that generate new proteins from ...
Het verschijnsel katalytische promiscuïteit, waarin een enzym naast zijn natuurlijke activiteit een ...
The genes (caaD1 and caaD2) encoding the trans-3-chloroacrylic acid dehalogenase (CaaD) of the 1,3-d...
Het verschijnsel katalytische promiscuïteit, waarin een enzym naast zijn natuurlijke activiteit een ...
The genes (caaD1 and caaD2) encoding the trans-3-chloroacrylic acid dehalogenase (CaaD) of the 1,3-d...
The tautomerization of 2-oxo-4E-hexendioate by 4-oxalocrotonate tautomerase has been studied by quan...
Tautomerase superfamily members have an amino-terminal proline and a beta-alpha-beta fold, and inclu...
Biocatalysis is a branch of biochemistry that exploits the ability of an enzyme to convert a substra...
The tautomerase superfamily (TSF) is a group of relatively short monomers (60-150 amino acid residue...
4-Oxalocrotonate tautomerase (4-OT) and trans-3-chloroacrylic acid dehalogenase (CaaD) are members o...
4-Oxalocrotonate tautomerase (4-OT) and trans-3-chloroacrylic acid dehalogenase (CaaD) are members o...
The enzyme 4-oxalocrotonate tautomerase (4-OT) is part of a catabolic pathway for aromatic hydrocarb...
The enzyme 4-oxalocrotonate tautomerase (4-OT) is part of a catabolic pathway for aromatic hydrocarb...
Enzymes are categorized into families based on sequence similarity and conserved features. Superfami...
textP[Kappa]a is not responsible for the improved activity. Hence, stabilization of an enediolate in...
Gene duplication and fusion are among the primary natural processes that generate new proteins from ...
Het verschijnsel katalytische promiscuïteit, waarin een enzym naast zijn natuurlijke activiteit een ...
The genes (caaD1 and caaD2) encoding the trans-3-chloroacrylic acid dehalogenase (CaaD) of the 1,3-d...
Het verschijnsel katalytische promiscuïteit, waarin een enzym naast zijn natuurlijke activiteit een ...
The genes (caaD1 and caaD2) encoding the trans-3-chloroacrylic acid dehalogenase (CaaD) of the 1,3-d...
The tautomerization of 2-oxo-4E-hexendioate by 4-oxalocrotonate tautomerase has been studied by quan...
Tautomerase superfamily members have an amino-terminal proline and a beta-alpha-beta fold, and inclu...