Immunohistochemical detection and quantification of myeloperoxidase, CD3 and CD45 positive cells in the lung during experimental AP.

(a) Immunohistochemistry for myeloperoxidase (MPO) in the lung after induction of acute pancreatitis and treatment with saline, azithromycin (AZM) or ampicillin (AMP). (b) Corresponding quantification of MPO+ positive cell. (c) Immunohistochemistry of CD3 in lungs of mice subjected to pancreatitis and treatment with saline, azithromycin (AZM) or ampicillin (AMP). (d) Corresponding quantification of CD3+ positive cells. (e) Immunohistochemical staining for CD45. (f) Corresponding quantification of CD45+ positive cells. Representative images are shown in 200x magnification. Scale bar represents 50 μm in all images. Data is shown as Whisker plots with indicated means of six individual animals from six independent experiments. n.s. = not significant. *p<0.05, n.s.: not significant. AZM: Azithromycin; SAL: saline; AMP: ampicillin; AZM: Azithromycin; SAL: saline; AMP: ampicillin.