Comparisons between the splenic lobe (SL) and duodenal lobe (DL) in relation to endocrine cell clustering during porcine pancreatic development.

(A–D, A’–D’) Morphological changes in the SL and DL in relation to endocrine cell clustering during porcine pancreatic development. The fluorescence micrographs show the expression of glucagon-positive cells (green) and insulin-positive cells (red). The nuclei are stained blue with Hoechst 33342. (A, A’) SL 28 days, (B, B’) DL 28 days, (C, C’) SL 35 days, and (D, D’) DL 35 days. The time after birth is represented as days. All of the panels are at the same magnification. Scale bars: 200 μm.