Effects of the C3U single nucleotide substitution on HCV genotype 1a virus production.

(A) Extracellular virus production. Huh7.5 cells were electroporated with 10 μg of infectious HCV genotype 1a (H77) RNA that does not (WT) or does contain the C3U mutation. Titers were determined by focus forming assays (FFU). Dotted line indicates threshold of detection. The C3U sample at day 22 may contain a revertant genome. (B) Virus titers of Huh7.5 cells infected with wild-type and C3U H77 variants at a multiplicity of infection (moi) of 0.005 and assayed at 72 hours post infection (**** p<0.0001). (C) RT-qPCR measurement of HCV viral RNA abundance at 5 days post-virus infection at an MOI of 0.005. Data normalized to internal control 18S rRNA levels (**** p<0.0001). Error bars display +/- SD.