Isolation and relative purity of sorted CD45-/Epcam-/ CD31+/Vegfr3+/Lyve1+/Pdpn+ PLECs.

Timothy A. Norman Jr. (6694004)
Adam C. Gower (190001)
Felicia Chen (6694007)
Alan Fine (456361)

Open link

Publication date

May 2019

DOI

10.1371/journal.pone.0216795.g002

Publisher

Public Library of Science (PLoS)

Abstract

(A, left) Cell sorting algorithm depicting three major cell populations; CD31-/Vegfr3- (grey), CD31+/Vegfr3- (yellow), CD31+/Vegfr3+ (purple) observed after excluding CD45+, Epcam+ and dead cells (E18.5 shown). (A, right) Expression of Lyve1 and Pdpn in each of the three cell fractions. (B) qRT-PCR measurements of relative Prox1, Flt4/Vegfr3, and Emcn expression in each sorted lung fraction. Statistical significance was determined using a two-tailed Student’s t test (***p.001, **p.01, *p.05; n = 3 per cell fraction).</p