Additional file 4: of Glutamine to proline conversion is associated with response to glutaminase inhibition in breast cancer

Figure showing HR MAS MRS data from untreated MAS98.06 and MAS98.12 tumors. a) Average 13C HR MAS MRS spectra calculated by subtracting natural abundance spectra from 13C enriched spectra. Positive signals with stars (*) indicate that there is a significantly higher amount of the metabolite in 13C-enriched samples compared to natural abundance samples, whereas up arrowheads (^) indicate borderline significance. b) Amount of 13C-labeled metabolites in the tumors, calculated by subtracting natural abundance spectra from 13C-enriched spectra. Stars (*) indicate that there is a significantly higher amount of the metabolite in 13C-enriched samples compared to natural abundance samples, and up arrowheads (^) indicate borderline significance. The total amount of 13C-labeled metabolites were not significantly different between the two models. c) Box plots showing the amount of the 13C-labeled metabolites subtracted with the amount of the metabolites from the natural abundance spectra. d) Amounts of selected metabolites from 1H spectra calculated from natural abundance and 13C-enriched samples. *p < 0.05, **p < 0.01, ***p < 0.001. Abbreviations: Ala, alanine; Gln, glutamine; GLS,: glutaminase; Glu, glutamate; Lac, lactate; Pro, proline; Pyr, pyruvate; TCA, tricarboxylic acid (PPTX 1939 kb