Differential mechanism of ERK1/2 activation in single versus double stimulation of B2R and TP in RASMC.

Cells were pretreated with the PKC inhibitor, Gö6983 (a), or the EGFR Tyr kinase inhibitor, AG1478 (b), prior to stimulation with BK, IBOP, or combination. Results are plotted as “fold/basal” ERK1/2 phosphorylation. Representative western blots are seen for pERK1/2 and tERK2. Results are mean ± SEM of at least three independent experiments. *: p < 0.05; as compared to unstimulated basal. #: p < 0.05; for pairwise comparisons, One Way ANOVA.