Age-dependent changes of the interscale differentiation marker.

(A) Schematic view of the interscale and scale IFE differentiation in the tail whole-mount epidermis. (B) Young and old tail epidermis stained with Keratin (K) 10 (differentiation marker of interscale, red), K31 (differentiation marker of scale, green) and Hoechst (blue). The greyscale images show K10 staining. Scale bars: 100 μm. (C, D) Quantification of the mean signal intensity of K10 (C) and K31 (D) from young and old tail whole-mount epidermis. N = 4. Error bars show S.E.M. Student’s t-test. ***; P P = 0.38 (D). (E) Young and old tail epidermis sections stained with K10 (red), K31 (green) and Hoechst (blue). The greyscale images show the K10 staining. Scale bars: 100 μm. (F, G) Quantification of the mean signal intensity of K10 (F) and K31 (G). The intensity of immunostaining signals in skin section is measured and averaged from ≥50 individual cells per mouse. N = 3. Student’s t-test. *; P P = 0.54 (G).