Physical and genetic maps of <i>L</i>. <i>tropica</i> hybrids.

A) Physical map showing the collective recombination breakpoints across the genome observed in 17 L.tropica hybrids generated between LtKub/SAT and Lt L747/HYG or LtMA37/NEO. The break points, represented by the red horizontal bars, are plotted by chromosome which are represented by the blue vertical bars that are roughly scaled to the chromosome lengths. Although the recombinations were distributed across the genome, there were apparent hotspots, defined as more than 2 recombinations in a 50kb window (highlighted by circles), and cold spots, including whole chromosomes, where few or no recombinations were found. B) A genetic map was constructed based on the crossovers observed in the 17 L. tropica hybrids. Centimorgan distances were calculated in windows of 20kb across the genome by counting total recombinations across all samples and dividing by total sample size. The total centimorgan distances for each chromosome are indicated at the top of the chromosome. The chromosome lengths are not true to scale, but are arranged from small to large as shown in Fig 6B. The total genome-wide map size is 2091.4 cM with an average of 61.5 cM/chromosome.