Histopathological findings in rats infected with <i>Anisakis</i> spp. L3.

(A) Severe submucosal hemorrhage (H) in stomach at the stomach-esophageal junction, adjacent to the site of larval penetration with two necrotic foci (asterisks) around large blood vessels (Bv) (HE, 100x); (a) inset: high-power magnification of a necrotic focus showing vascular and perivascular necrosis, surrounded by abundant neutrophil and macrophage infiltrate (arrows) (400x); (B) Stomach of a rat euthanized 24 h p.i. with abundant eosinophil infiltrate in lamina propria and around blood vessels (Bv) (arrowhead) indicating recent extravasation (HE, 100x); (b) inset: detail of abundant eosinophil infiltrate (400x); (C) Severe submucosal hemorrhage (H) in intestine with a cross-section of Anisakis spp. larva (L3) (HE, 100x); (c) inset: detail of mucosa with rare eosinophils in lamina propria (arrowheads) (HE, 400x); (D) Extensive submucosal hemorrhage (H) in caecum (HE, 100x); (d) inset: detail of mucosa with abundant eosinophil infiltration (arrowheads) in lamina propria (HE, 400x); (E) Migration of Anisakis spp. larvae (L3) through epimysial connective tissue (Epm) causing moderate to extensive hemorrhage accompanied by mixed inflammatory infiltrate, composed mostly of neutrophils and macrophages (Mf = muscle fibers) (HE, 100x); (F) Muscle tissue adjacent to site of larval migration showing mild perimysial hemorrhage (asterisks) and focal necrosis of muscle fibers (Mf) (HE, 100x); (f) inset: detail of perimysial connective tissue with two blood vessels (Bv) surrounded by moderate neutrophil infiltrate (arrow) (400x). Scale bar (A, B, C, D, E, F) = 200 μm, scale bar (inset) = 50 μm.