Representative micrographs of S100A8/A9 (MRP8+MRP14) immunolabeling in rats experimentally infected with <i>Anisakis</i> spp. third-stage larvae.

(A) Strong diffuse cytoplasmic labeling of S100A8/A9 in a low number of cells in the inner layer of the muscularis externa of rat stomach (arrows); (B) DAPI-stained cell nuclei; (C) Merge of (A) + (B); (D) Moderate diffuse cytoplasmic expression of S100A8/A9 in endothelial cells in intestinal submucosa; (E) DAPI-stained cell nuclei; (F) Merge of (D) + (E); (G) Very strong sarcoplasmic expression of S100A8/A9 in abdominal muscle myocytes (arrows); (H) DAPI-stained nuclei; (I) Merge of (G) + (H). Immunofluorescence, scale bar = 10 μm.