Limited HIV-2 reservoirs in central-memory CD4 T-cells associated to CXCR6 co-receptor expression in attenuated HIV-2 infection - Fig 5

A)In vitro inducibility of HIV-2 reservoir in CD8-depleted peripheral blood mononuclear cells (PBMCs). Cell capacity to induce HIV-2 replication was evaluated in 11 HIV-2 infected subjects by stimulating CD8-depleted PBMC with anti-CD3/anti-CD28 co-stimulation plus IL-2, plus IL-7 plus IL-15. Allogeneic feeder CD8-depleted PBMC from a donor were added to culture at D13. Culture supernatants were collected every three days for HIV-2 viral load quantification from D0 to D31. Results are expressed as the log10 of the ratio between the numbers of HIV-RNA copies quantified on a given day of culture and the level of total HIV-DNA in the subset measured at D0 of culture. Each symbol represents a subject. B) Expression of CCR5 in culture on CD4 T-cells from 7 HIV-2 infected subjects (dot) compared to 7 donors (D) (square). Median expression of CCR5 on CD4 T-cells during 14 days of culture is represented.