MOESM1 of Susceptibility to L. sigmodontis infection is highest in animals lacking IL-4R/IL-5 compared to single knockouts of IL-4R, IL-5 or eosinophils

Additional file 1: Figure S1. Representative gating strategy using thoracic cavity cells of a wildtype mouse 71 dpi. Lymphocytes (P3) were assessed for their expression of CD4 and CD8 to identify CD4+ and CD8+ T cells. All cells (P1) were analysed to differentiate eosinophils (SiglecF+ F4/80-) and macrophages (SiglecFlow/int F4/80+). SiglecF- F4/80- cells (P2) were analyzed for their expression of Gr-1 to identify neutrophils. Gating of RELMÎą+ macrophages was performed using the fluorescence minus one (FMO) approach