Effect of DEX on the PI3K/AKT pathway and ERK1/2 pathway.

The activation status of the activating kinases AKT and ERK1/2 was investigated in WT and AT cells. A),C) Western blot analysis for phosphorylated AKT/total AKT and phosphorylated ERK1/2/total ERK1/2, respectively, in WT (238RM) and AT (AT28RM, AT50RM, AT129RM) cells treated with 100 nM DEX for 24 hours or not treated. B),D) p-AKT and p-ERK1/2 abundance was quantified and normalized, respectively, by the value of total AKT and total ERK1/2 signal. Blots shown are representative and values are the means and SEM of four independent experiments (Wilcoxon signed rand test; *two-tailed p-values<0.05).