Simulated-field and semi-field evaluation of syt.427 activity.

Activity of syt.427 was confirmed in assays conducted with rainwater (A; data shown are from replicate experiments that included a total of 120 larvae per treatment) and using larvae from a field strain of A. aegypti mosquitoes generated from eggs collected in Trinidad (B; data shown were combined from replicate experiments that included a total of 240 larvae per treatment). High levels of larval mortality were induced by syt.427 in semi-field trials (C) conducted in a contained outdoor roof top laboratory (D). The semi-field data shown (C) were compiled from multiple biological replicate experiments conducted on a total of 180 larvae/treatment. Data are represented as mean percentage larval mortality and were evaluated with t-tests; error bars denote SEM. ***P<0.001 when compared with control-fed larvae.