<i>Wolbachia</i> inhibits infection with ZIKV and DENV-3 during co-infection.

Wildtype (WT, circles) and wMel-infected (Mel, diamonds) A. aegypti were orally challenged with both ZIKV (blue) and DENV-3 (orange), with the viruses offered at approximately equivalent titres. Mosquitoes were collected at 7, 14 and 21 days post-infection, and disseminated infection levels were determined for individual head/thorax samples via RT-qPCR using specific TaqMan probes. Mel mosquitoes displayed significantly higher proportion of uninfected mosquitoes than WT at all three time points (A). Mel mosquitoes had significantly lower ZIKV (B) and DENV-3 (C) load than WT mosquitoes. Data represent two replicate experiments. Pie charts depict prevalence of infection, with P values determined via Chi-square test. Dot plots depict viral load for infected samples only, with P values determined via Mann-Whitney U test for all comparisons where there were sufficient numbers of virus-infected Mel samples. Black bars in the dot plots represent treatment medians. Each dot represents a single mosquito sample. N = 34–40 samples per treatment.