Checkpoint inhibitors increase the number of T cells in the liver of <i>PD-1</i><sup><i>-/-</i></sup> mice.
- Timothy Affolter (1593382)
- Heather P. Llewellyn (6718565)
- Derek W. Bartlett (61130)
- Qing Zong (428068)
- Shuhua Xia (1743688)
- Vince Torti (6718568)
- Changhua Ji (48504)
Publication date
May 2019
DOI Publisher
Public Library of Science (PLoS)Abstract
Mice were treated with anti-mouse CTLA-4 (9D9), lower dose (300 mg/kg BID) epacadostat or in combination for 6 weeks (A–H) or 3 weeks (I). Single cell suspensions of the whole liver were analyzed for (A) CD45+ cells, (B) CD11b+F4/80+ macrophages, (C) Gr1+CD11b+ myeloid derived suppressor cells (MDSC)s, (D) CD49b+ natural kill (NK) cells, (E) CD3+ T cells, (F) CD3+CD8+ T cells (G) CD3+CD4+ T cells, (H) Foxp3+CD4+ T cells, and (I) Ki67+ T cell subsets by flow cytometry. All data are reported as mean ± SEM. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001, n = 6 per group, one-way ANOVA with Tukey’s multiple comparisons test (A–H) or n = 12 per group, unpaired t-test (I).</p
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