Four representative fluorochrome-conjugated antibody titrations.
- Elvira Jimenez Vera (6725789)
- Yi Vee Chew (6725792)
- Leigh Nicholson (6725795)
- Heather Burns (6725798)
- Patricia Anderson (6725801)
- Hsiao-Ting Chen (6725804)
- Lindy Williams (6725807)
- Karen Keung (6725810)
- Negar Talaei Zanjani (6725813)
- Suat Dervish (6725816)
- Ellis Patrick (4219258)
- Xin Maggie Wang (6725819)
- Shounan Yi (510456)
- Wayne Hawthorne (6725822)
- Stephen Alexander (452429)
- Philip J. O’Connell (6725825)
- Min Hu (41912)
Publication date
May 2019
DOI Publisher
Public Library of Science (PLoS)Abstract
(A) Concatenated pseudocolor data at 5–6 antibody concentrations for APC-CD8 (RPA-T8), PE-CF594-CD19 (HIB19), BB515-CD25 (2A3) and APC-CD154 (TRAP1). (B) Staining indexes and antibody concentrations for APC-CD8, PE-CF594-CD19, BB515-CD25 and APC-CD154. APC-CD8, PE-CF594-CD19, BB515-CD25 were assessed on lymphocytes (excluding CD4+ cells), lymphocytes or CD3+CD4+ T cells respectively. APC-CD154 was evaluated on CD3+CD4+ T cells of PBMCs that were activated with CD3/CD28 beads overnight. The final antibody concentrations used in the study for APC-CD8, PE-CF594-CD19, BB515-CD25 and APC-154 were 0.05 μg/ml, 0.38 μg/ml, 0.83 μg/ml, 0.3 μg/ml respectively.</p
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