CKII site mutant C4-1 cells show defects in matrigel invasion.

Equal numbers of wild type and mutant C4-1 cell lines were seeded in the upper part of Matrigel invasion chambers, in DMEM without serum. DMEM with 2% serum was used as a chemoattractant in the lower chamber. After 22h, cells that had invaded the lower chamber were fixed and stained with 0.5% Crystal Violet in 5% glutaraldehyde for 10 mins. (A) Representative micrographs showing invasive cells from each cell line as indicated. (B) Quantification of the mean number invasive cells shown as bars with the standard error of mean. Asterisks indicate the p-value of mean numbers of invasive cells, compared with the wild type cells.