Additional file 3: of Identification of dynamic glucocorticoid-induced methylation changes at the FKBP5 locus

Figure S3. CpG sites with significant genotype-dependent dynamic methylation differences in both studies. Effects of rs1360780 genotype on DEX-induced DNA methylation changes in 2 sites located in intron 7 and 5 enhancers. The % methylation levels for rs1360780 risk allele carriers CT/TT and CC carriers following DEX exposure are shown for each study. Methylation of CpG 35558710 shows significant interaction effect at 23 h in study 1 (Χ2 = 5.69, p value = 0.02) and additive effect at 3 h in study 2 (Χ2 = 4.15, p value = 0.04) with risk allele genotype. Significant interactions between risk allele genotype and DEX on methylation were observed for CpG 35570224 at 6 h and 23 h post-treatment in study 1 (Χ2 = 7.59, p value = 0.006 and Χ2 = 6.0, p value = 0.01) and at 24 h in study 2 (Χ2 = 4.36, p value = 0.04). Points and error bars represent mean and SEM for each genotype. (PDF 101 kb