Sensitivity analysis of <i>fancjb-1 rad5A-2</i>, <i>fancjb-1 rev3-5</i> and <i>fancjb-1 mus81-1</i> double mutants in response to MMC treatment.

Relative fresh weight of double mutants in comparison to the corresponding single mutants and wild-type plants (WT) was determined after MMC treatment. At least 4 independent assays were performed and mean values with standard deviations (error bars) were calculated. (A) The relative fresh weight of fancjb-1 rad5A-2 double mutants was comparable to the rad5A-2 single mutant in all tested MMC concentrations. (B) The fancjb-1 rev3-5 double mutant exhibited a relative fresh weight that was not significantly different from the rev3-5 single mutant after treatment with MMC. (C) In fancjb-1 mus81-1 double mutants, a significantly reduced fresh weight compared to both single mutants could be observed after 5 and 10 μg/ml MMC treatment. Statistical differences were calculated using the two-tailed t-test with unequal variances: * p < 0.05, ** p < 0.01, *** p < 0.001, ns = not significant.