Effect of SMI on cell viability and clonogenicity of NSCLC cell lines.

NSCLC cell lines were treated with different concentrations of SMI (10 to 80 μM). (A) number of viable cells were expressed as % untreated cells counted by trypan blue exclusion technique in SMI treated and shRNA-PKM2 silenced 3 normal lung and 11 NSCLC cell lines. (B) For clonogenic assay, cells were incubated for 8 days to form colonies. Number of colonies was counted. (C) Number of colonies formed by NSCLC cells treated with different concentrations of SMI and shRNA PKM2 were stained with 0.25% crystal violet in 25% ethanol, washed with water and air dried.