NCTD inhibits cell growth <i>in vitro</i> and <i>in vivo</i>.

(A, B) MTT assays of cell proliferation in MCF-7 (A) and ZR75-1 (B) cells in the presence of 25μM NCTD. (C) NCTD inhibited tumor growth in a human breast cancer MCF-7 xenograft mouse model. Tumor weights were calculated and shown as a box-plot with median and whiskers from minimum to maximum. (D) NCTD inhibited cell growth via miR-873/CDK3 axis. MCF-7 cells were transfected with anti-miR-873 oligo or CDK3 expression vector and then subjected to 25μM NCTD and 100nM E2 treatment for 6 days. MTT assay was performed to detect the cell proliferation. Data are expressed as mean ± SD. * P < 0.05.