Physical events occurring during the cryopreservation of immortalized human T cells - Fig 2

A typical DSC trace of Jurkat cells (solid lines) and cell-free supernatant (dotted lines) during warming, and their first-order (grey) and second-order (black) derivatives. Both samples were separated by centrifugation from a suspension of Jurkat cells in culture medium with 10% (v/v) dimethyl sulfoxide. They were initially cooled at 2°C min-1 to -150°C. Three distinct thermal events are apparent: the extracellular glass transition (A), the intracellular glass transition (B), and bulk melting of the extracellular solution (C). Note that transition B, characterized by its onset, maximum and endset, is absent in the DSC trace of the cell-free supernatant.