H69 cell sprouting into COL1 hydrogels with differing mechanical properties.

(A) Sprouting of H69 cells under various ECM conditions; general control (2.0 mg/mL of COL1, pH 7.4), higher pH (2.0 mg/mL, pH 11), and higher concentration (2.5 mg/mL, pH 7.4). (B) Quantification of cell sprouting of H69 cells into COL1 hydrogels (area). Compared to the general control, there were no significant differences between groups. Scale bars = 100 μm (A). Student’s t-test was used to analyze significance. Error bars = ± SEM.