Increased p-AMPK, p-Akt, and p-eNOS in the aorta of EX mice.

Equal amounts of aortic homogenate were separated by SDS-PAGE. Representative blots are shown above each summary graph. A. Phospho-AMPKα (Thr172) was normalized to GAPDH expression and expressed relative to SED mean (n = 7 per group). *pB. Phospho-Akt (Ser473) was normalized to GAPDH expression and expressed relative to SED mean (n = 7 per group). *pC. Phospho-eNOS (Ser1177) was normalized to GAPDH expression and expressed relative to SED mean (n = 7 per group). *pD. Total eNOS protein expression was normalized to GAPDH and expressed relative to SED mean (n = 7 per group). *p<0.001.