Detailed analysis of primary root cell length after treatment with TkRALFL1.

(A) Root tip image acquired by CLSM of a representative seedling treated for 10 days with non-peptide control. Seedlings were stained with propidium iodide (0.1 mM). The white arrow indicates the distance of 1 mm from the very tip; scale bar as indicated in the respective images; the black bar above the magnified segment indicates the most inner six rows of rhizodermal cells measured for evaluation of the cell length, one cell is exemplarily marked by a white bar. (B) Root tip image of a representative seedling treated for 10 days with 0.1 μM His-TkRALFL1 as described in Fig 3A. (C) Cell length of rhizodermal cells from root tips of seedlings treated for 10 days with different concentrations of TkRALFL1 as indicated. n = 30, normal distribution proved by Kolmogorov-Smirnov test, statistical significance proved by two-tailed t-test, *pFig 2.