A botanical survey of the vascular flora of the planning and zoning jurisdiction of the city of Ames, Iowa (i.e., the area within a boundary 3.2 km beyond the current city limits) was compiled from 1990 to 2000. During this survey, 916 taxa (71 % native) were encountered within chis boundary. Literature reviews and a survey of Iowa State University\u27s Ada Hayden Herbarium for specimens that had been collected in Ames since 1859 add 204 taxa to the flora. This total of 1,120 taxa exceeds the number of taxa known from any comparable area (including counties) in Iowa. We produced a checklist including date of first record, origin, abundance and habitat codes for all species that were noted during the current survey. Information for histori...