A Standardized Method for Evaluating the Image in Fluorescence Microscopy

The problem of evaluating the virtual image in fluorescence microscopy is essentially one of assaying the several attributes of color, viz. hue, value, and chroma or saturation. DeMent (1942) suggested that fluorescence phenomena be reported according to the recommendations on color of the American Optical Society. This is a qualitative estimate employing some twenty-one hue names qualified by eight adjectives. The present work has been designed to delimit these adjectives and to provide a common denominator for the concept of hue. The concept of hue varies not only with an individual\u27s inherited ability to perceive color, but also with the extent of his training in the observation of color. Many people are not color blind, but are color ignorant, which is to say that their ideas of color are not the same as those of the person who classifies them as color ignorant. What is bright or dull, or light or dark, to one person is not necessarily the same to another. What is pink, or orange, or blue to one person may not be the same to another, at least not to the same degree. Consequently, there is a multiplicity of terms in the nomenclature of color. Because of this it is desirable to employ a color standard which takes these variations into account and to adapt its use to the problem at hand