Rabbit muscle glyceraldehyle-3-phosphate dehydrogenase (GAPD) was dissociated into a subunit species via lauryl sulfate (LS). The incubation mixture prepared from active GAPD 10-5 M and LS 10-3 M showed a rapid loss of initial activity. Within 30 minutes after the addition of the powdered LS, there was a 50 percent loss in initial enzymatic activity. The appearance of the subunit species was verified by Sephadex (a crossed-linked dextran) molecular sieve studies. Using a column buffer containing 0.01 M acetic acid, 0.10 M NaCl and 0.005 M LS at 25° C., with a pH of 6.1 ± 0.1, elution patterns of GAPD, conalbumin, hemoglobin and lysozyme were used to calibrate a G-100 Sephadex column. From the Sephadex gel filtration studies, it was discerne...
AbstractRabbit muscle glyceraldehyde-3-phosphate dehydrogenase covalently bound to Sepharose was sho...
Phosphorylase kinase activity is renatured and detected in situ following electrophoresis of the den...
1. 1. A molecular sieve membrane was used to separate active dimer vs active tetramer fractions of M...
Rabbit muscle glyceraldehyle-3-phosphate dehydrogenase (GAPD) was dissociated into a subunit species...
Frontal gel chromatography and sedimentation studies have been used to establish that rabbit muscle ...
We have isolated a protein from the soluble fraction of skeletal muscle which stimulates the formati...
AbstractIncorporation of l-[35S]cysteine into rabbit muscle glyceraldehyde-3-phosphate dehydrogenase...
The rabbit muscle is one of the most frequently used sources for glyceraldehyde-3-phosphate dehydrog...
AbstractIncubation of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) with micromolar hydrogen pero...
The enzyme glyceraldehyde-3-phosphate dehydrogenase E.G.1.2.1.12 has been prepared from rabbit muscl...
A protein of subunit Mr 34,000 (corrected here and subsequently to 36,000) has been isolated from a ...
ABSTRACT Theexceptionallyhighprotein con-centration in living cells can favor functional pro-tein–pr...
Glucose‐6‐phosphate dehydrogenase from human erythrocytes shows successive aggregation‐dissociation ...
AbstractGymnemic acids (GA) inhibited rabbit muscle glyceraldehyde-3-phosphate dehydrogenase (GAPDH)...
AbstractOne of the 3 major RNA-binding proteins of rabbit reticulocytes, a polypeptide of 36 kDa, is...
AbstractRabbit muscle glyceraldehyde-3-phosphate dehydrogenase covalently bound to Sepharose was sho...
Phosphorylase kinase activity is renatured and detected in situ following electrophoresis of the den...
1. 1. A molecular sieve membrane was used to separate active dimer vs active tetramer fractions of M...
Rabbit muscle glyceraldehyle-3-phosphate dehydrogenase (GAPD) was dissociated into a subunit species...
Frontal gel chromatography and sedimentation studies have been used to establish that rabbit muscle ...
We have isolated a protein from the soluble fraction of skeletal muscle which stimulates the formati...
AbstractIncorporation of l-[35S]cysteine into rabbit muscle glyceraldehyde-3-phosphate dehydrogenase...
The rabbit muscle is one of the most frequently used sources for glyceraldehyde-3-phosphate dehydrog...
AbstractIncubation of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) with micromolar hydrogen pero...
The enzyme glyceraldehyde-3-phosphate dehydrogenase E.G.1.2.1.12 has been prepared from rabbit muscl...
A protein of subunit Mr 34,000 (corrected here and subsequently to 36,000) has been isolated from a ...
ABSTRACT Theexceptionallyhighprotein con-centration in living cells can favor functional pro-tein–pr...
Glucose‐6‐phosphate dehydrogenase from human erythrocytes shows successive aggregation‐dissociation ...
AbstractGymnemic acids (GA) inhibited rabbit muscle glyceraldehyde-3-phosphate dehydrogenase (GAPDH)...
AbstractOne of the 3 major RNA-binding proteins of rabbit reticulocytes, a polypeptide of 36 kDa, is...
AbstractRabbit muscle glyceraldehyde-3-phosphate dehydrogenase covalently bound to Sepharose was sho...
Phosphorylase kinase activity is renatured and detected in situ following electrophoresis of the den...
1. 1. A molecular sieve membrane was used to separate active dimer vs active tetramer fractions of M...