The importance of Mollicutes in urological infections

The bachelor thesis deals with the importance of the Mollicutes class in urological infections. It is focused on the diagnosis of urogenital mycoplasma, namely Mycoplasma hominis and Ureaplasma urealyticum. Mollicutes form a unique group of microorganisms that represent the smallest known organism capable of an autonomous life. Mycoplasma and Ureaplasma are of the greatest importance for humans. They are most commonly found in the oral cavity, in the respiratory tract and in the urogenital tract. The most well-known mycoplasmas that cause urogenital tract disease are Mycoplasma hominis and Ureaplasma urealyticum. The aim of the theoretical part of the thesis was to gather available scientific literature on this topic, including Internet resources. The aim of the practical part was to master the laboratory examination of the urological material to Mollicutes class, namely Mycoplasma hominis and Ureaplasma urealyticum strains. In order to achieve this goal, the results of a long-term research investigation of the laboratory from the Czech Republic and the Slovak Republic were collected. The bachelor thesis also compared laboratory results from the Czech Republic, Kladno and Bratislava. Results were obtained using two different methods, the culture kit and the PCR method. The results of the investigation showed that the Ureaplasma urealyticum strain occurs much more frequently than the Mycoplasma hominis strain. Summary results are consistent with the literature on the incidence of urogenital mycoplasma. During the elaboration of my bachelor thesis, I learned to manually handle the methods of the theoretical part of my thesis, including isolation of DNA mycoplasmas and ureaplasmas. The results provide information on urogenital infections, their diagnosis, treatment and prevention, thus this work can provide information for healthcare professionals, health care students, or others interested in this theme