Detection of Ralstonia solanacearum bacteria pathogenic for tomato, pepper and potato by loop-mediated isothermal amplification

For rapid detection of quarantine bacteria Ralstonia solancearum pathogenic for tomato, potato and papper, the LAMP method was used. Based on the analysis of the fliC gene sequence, a set of LAMP primers was designed to be specific for Ralstonia solanacearum. Subsequently, the method was optimized to detect only Ralstonia solanacearum and to distinguish it from the other phytopathogenic bacteria which infect potato, tomato and pepper. LAMP can detect bacteria from very small amounts of DNA in less than 30 minutes in real time. The method is capable of amplifing DNA at a concentration of 0.00001 ng/?l. However, a higher DNA concentration is more suitable for more specific detection