ANTIMICROBIAL ACTIVITY OF SPONGE-ASSOCIATED FUNGI FROM PANDANG ISLAND, NORTH SUMATERA AGAINST CLINICAL PATHOGENIC MICROORGANISMS

As an archipelago and maritime country, Indonesia is consisted by numerous small islands with abundance of marine organisms. Pandang Island is one of the outer islands in Indonesia, located in Batu Bara District, North Sumatera Province. The aims of this study were to isolate the marine sponge associated fungi, obtain potential strain against clinical pathogenic fungi and MDR bacteria, determine the best cultivation time for antimicrobial production, characterize potential fungus through macromicroscopic and molecular approaches. The result showed there were 6 fungi which associated with sponge PPD.SP.7 and only fungus PDSP 7.1 could inhibit all pathogenic microorganisms. This fungus produced widest inhibition zone against S. enterica ser. Typhi in stationary phase. According to the result of identification, fungus PDSP 7.1 was judge as Trichoderma saturnisporum MG644629. Antimicrobial activity of this fungus was expected as the influence of the presence of NRPS gene fragments. This research is a first report which studied sponge associated fungi and its antimicrobial activity from Pandang Island, North Sumatera, Indonesia