Determination of glucose using polyaniline modified electrode

Sensor electrode was formed by immobilization of glucose oxidase (GOx) on polyaniline (PANI), electrochemically synthesized on graphite electrode from aqueous hydrochloride acid electrolyte containing aniline monomer by galvanostatic method. Optimization of the current density used for the synthesis of PANI was performed. Immobilization of GOx was achieved by crosslinking via glutaraldehyde and the efficiency of the immobilization was determined spectrophotometrically. Using chronoamperometric curves of glucose oxidation on polyaniline apparent Michaelis constant was estimated to be 0.273 mM. The storage stability of the enzyme electrode was examined for twenty days, after which it retained 84% of its initial signal