Combination of standardized ecotoxicological tests and in situ measurements to characterize the effects of an effluent from a bio-refinery on a freshwater ecosystem

The chemical industry is a very important economic sector in France with used especially oil and their derivates. Nevertheless, the growing concern of economic world and population contributed to the development of green chemistry notably based on utilization of the biomass. The development of this new type of industry is mediated through the generation of bio-refinery. These bio-refineries are able to transform the agricultural and forest production to bio-energy and bio-product. The present work aims to better understand the ecological and environmental impact of green chemistry on freshwater ecosystem. In this context, some fish and shellfish were caged during 21 days upstream and downstream a bio-refinery of derived glycerin in order to identify potential immunotoxic and genotoxic effects. In the same way, the environmental impact of bio-refinery effluent was characterized in a regulatory context using standardized ecotoxicity tests (e.g mobility of Daphnia magna, growth of the unicellular green algae Pseudokirchneriella subcapitata, reproduction of Ceriodaphnia dubia). The results of this combined approach will be discussed in the poster