Synthetic dyes decolourisation on agar plates by selected fungi

U ovom radu istražena je sposobnost obezbojenja sintetskih bojila (malahitno zelenilo MZ, kristal violet KV, fuksin F i metilensko modrilo MM) pomoću četiri vrste gljiva bijelog truljenja: P. chrysosporium CCBAS 570, T. versicolor CCBAS AG613, T. versicolor TV6 i C. subvermispora. Gljive su uzgajane pri 27 ⁰C 9 (10) dana na agarnim pločama s dodatkom bojila u koncentracijama 50, 100 i 150 mg L\(^{-1}\). Zona rasta kolonija te zona obezbojenja (promjene boje) mjerene su (u dva međusobno okomita smjera) svaka 3 dana. MZ je snažno inhibiralo rast svih vrsta, ali su sve pokazale dobru sposobnost obezbojenja ovog bojila, što je vidljivo iz velikog indeksa obezbojenja (promjer obezbojenja/promjer kolonije) u rasponu od 2,42 do 6,04. Sposobnost djelomičnog obezbojenja KV pokazale su sve vrste gljiva, pri čemu je rast vrsta P. chrysosporium i C. subvermispora bio snažno inhibiran ovim bojilom. F je inhibirao rast odabranih gljiva samo tijekom prvih dana uzgoja. Sposobnost obezbojenja F pokazale su sve vrste, ali indeks obezbojenja nije bio velik (0,24 – 1). Inhibicija rasta ovim bojilom primijećena je samo u prvim danima uzgoja. MM nije inhibiralo rast gljiva, ali je djelomičnu sposobnost obezbojenja ovog bojila pokazala jedino vrsta T. versicolor TV6. Utvrđeno je kako primijenjene koncentracije bojila u podlozi statistički značajno utječu na rast kolonija gljiva, ali nije utvrđena statistički značajna razlika u sposobnosti obezbojenja.Four white rot fungi P. chrysosporium CCBAS 570, T. versicolor CCBAS AG613, T. versicolor TV6 and C. subvermispora were tested for their ability to decolourise synthetic dyes (Malachite Green MG, Crystal Violet CV, Fuchsine F and Methylene Blue MB). Fungal species were cultivated at 27 °C for 9 (10) days on Potato dextrose agar plates containing dyes at final concentrations of 50, 100 and 150 mg L\(^{-1}\). The colony radial growth and the decolourisation zone (in two perpendicular directions) were measured every three days. Although all fungal strains were strongly inhibited by MG, high decolourisation index (decolourisation diameter/mycelial diameter) ranging from 2.42 to 6.04 for all tested species indicated very good decolourisation ability. CV strongly inhibited the growth of all fungi except P. chrysosporium. However, all species partially decolourised CV. F was also decolourised by all strains but the decrease in decolourisation efficiency was observed (decolourisation index ranging from 0.24 to 1). Inhibitory effect of F to fungal growth was observed only during the first cultivation days. Only T. versicolor TV6 partially decolourised MB, even though this dye did not inhibit the mycelial growth of the tested fungal strains. The influence of agar plate dye concentration on fungal growth was statistically significant, while no statistical significance was observed regarding the decolourisation ability