The Essential Role of Oct-2 in Lps-Induced Expression of Inos in Raw 264. 7 Macrophages and Its Regulation by Trichostatin A

The essential role of Oct-2 in LPS-induced expression of iNOS in Raw 264.7 macrophages and its regulation by trichostatin A. Am J Physiol Cell Physiol 296: C1133-C1139, 2009. First published March 11, 2009; doi: 10.1152/ajpcell.00031.2009.-This article reports on a study of the effect of trichostatin A (TSA), an inhibitor of histone deacetylase, on lipopolysaccharide (LPS)-induced expression of inducible nitric oxide synthase (iNOS) in RAW 264.7 macrophages and its underlying mechanisms. TSA pretreatment potently diminishes LPS-stimulated nitric oxide (NO) release and both mRNA and protein levels of iNOS in macrophages. The effects of TSA and LPS on transcription factors binding to two LPS-responsive elements within the iNOS promoter, one binding the NF-kappa B site and the other the octamer element, were investigated. Results show that TSA did not alter the LPS-activated NF-kappa B activity demonstrated by the nuclear translocation of p50 and p65 and by a NF-kappa B-driven reporter gene expression system. In addition, neither TSA nor LPS changed the expression of Oct- 1, a ubiquitously expressed octamer binding protein. However, TSA suppressed the LPS-induced expression of Oct -2, another octamer binding protein, at both mRNA and protein levels. Chromatin immunoprecipitation assays revealed that binding of Oct-2 to the iNOS promoter was enhanced by LPS treatment; however, pretreatment with TSA resulted in loss of this binding. Moreover, forced expression of Oct-2 by transfection of pCG-Oct-2 plasmid restored the TSA- suppressed iNOS expression elevated by LPS stimulation, further indicating that Oct-2 activation is a crucial step for transcriptional activation of the iNOS gene in response to LPS stimulation in macrophages. This study demonstrates that TSA diminishes iNOS expression in LPS-treated macrophages by inhibiting Oct-2 expression and thus reducing the production of NO