Studies on the Chemical Constituents from Tradescantia albiflora Kunth

白花水竹草 (Tradescantia albiflora Kunth ) ，為直立或匍匐性的多年草，原產於北美與南美，1975年間從法國引進台灣。白花水竹草具有降低血中尿酸的功能，並有抗氧化的效果。論文為研究白花水竹草的化學成分，先以甲醇浸泡得到萃取物，利用矽膠管柱層析、矽膠薄層層析、高效液相層析 (HPLC) 及再結晶等方法進行分離純化。純化後得的化合物，再利用氫核磁共振圖譜、碳核磁共振圖譜、紅外線光譜與質譜來推測結構。若推測的結構，經化學資料庫查詢為已知化合物，則比較文獻確定其結構；若為新化合物，則以二維核磁共振圖譜 (2D-NMR) 及高解析質譜的輔助，來進行結構的鑑定。實驗共鑑定得到40個化合物，分類為:、醯胺類 ( Amides ) 化合物2個、維他命A代謝物類 ( Apocarotenoids ) 化合物7個、芳香族類 ( Aromatics ) 化合物13個、葉綠素類 ( Chlorophylls ) 化合物1個、類黃酮類 ( Flavonoids ) 化合物1個、固醇類 ( Steroids ) 化合物7個、三萜類 (Triterpenoids) 化合物2個、其他類 ( Others ) 化合物7個中有3個新化合物。Tradescantia albiflora Kunth is a species of spiderwort. In the past it was considered an invasive species or pest plant and was consequently targeted for eradication. Recently, it was found T. albiflora can decrease the level of blood uric acid.he whole plants of of T. albiflora were extrated with methanol. The extract was concentrated in vacuo to yield the residue which was suspended in water, and then partitioned with ethyl acetate. The ethyl acetate layer was separated by column chromatography on silica gel, preparative thin layer chromatography and high performance liquid chromatography (HPLC) to obtain 40 compounds, including 2 amides, 7 apocarotenoids, 13 aromatics, 1 chlorophyll, 1 flavonoid, 7 steroids, 2 triterpenoids and 7 other compounds. Among them, 3 compound are new compounds.he known compounds were identified by spectral analysis and compared with those reported in literatures or from authentic samples. The structures of new compounds were elucidated by spectral evidence including 1D and 2D NMR techniques.口試委員會審定書誌.......................................................I中文摘要..................................................II英文摘要.......................................................III第一章 緒論一節 研究目的...........................................1二節 植物簡介...........................................1三節 白花水竹草之研究概述...............................2二章 結果與討論一節 分離程序與結果.....................................4二節 維他命A代謝物類化合物結構鑑定.....................13化合物AP7結構鑑定.................................14三節 芳香族類化合物結構鑑定............................21化合物AR10結構鑑定................................22化合物AR11結構鑑定................................30化合物AR12結構鑑定................................38化合物AR13結構鑑定................................46三章 結論一節 化合物骨架分析....................................54第二節 化合物骨架之生合成推演....................55四章 實驗部份第一節 實驗儀器及藥品............................61第二節 實驗步驟..................................62第三節 已知化合物數據............................66五章 參考文獻..........................................7