Optimization of Micellar Electrokinetic Chromatographic Separation of Aporphine Alkaloids Using the Overlapping Resolution Mapping Scheme

Based on previous work using the univariate approach to optimize the separation of nine Lauraceous aporphine alkaloids, lindcarpine, laurolitsine, N-methyllindcarpine, boldine, norpredicentrine, isocorydine, laurotetanine, N- methyllaurotetanine, and isoboldine, by micellar electrokinetic chromatography, a three-parameter overlapping resolution mapping (ORM) scheme was employed to optimize the separation of these compounds. With the other conditions preoptimized, three buffer parameters , i.e., the concentrations of sodium borate, sodium dodecylsulfate, and sodium heptanesulfonate were chosen to carry out the optimizatio process. Conditions were identified that allowed base-line separation of the nine aporphines and the internal standard within eight minutes. These results shows that ORM is an efficient optimization procedure, although some primary separation data is first required