Factor V Arg306->Gly Mutation Is Not Associated with Activated Protein C Resistance and Is Rare in Taiwanese Chinese

Polymerase chain reaction amplification followed by BstO1 enzyme digestion and DNA sequencing was employed to detect the mutation of factor V gene. The subjects consisted of 105 venous thrombophilic patients and 183 healthy controls. Only one patient was found to have factor V Arg306-> Gly mutation, his elder son also had an identical mutation. None of the healthy subjects studied had Arg306->Thr mutation. The rare event of factor V Arg306->Gly mutation in patients and controls suggest that this mutation is not associated with increased risk of venous thrombosis. Conventional, modified and extended activated protein C (APC) resistance assay in this patient and his family members clearly showed that factor V Arg306->Gly mutation is not associated with APC resistance (APC sensitivity ratio Gly mutation is rare in Taiwanese Chinese and not associated with APC resistance, it is possibly not a risk factor for venous thrombophilic thrombosis