Previous structural and mutational studies of bovine pancreatic deoxyribonuclease I (bpDNasc I) have demonstrated that the active site His 134 and His252 played critical roles in catalysis. In our present study, mutations of these two His residues to Gin, Ala or Gly reduced the DNase activity by a factor of four to five orders of magnitude. When imidazole or primary amines were added exogenously to the Ala or Gly mutants, the residual DNase activities were substantially increased by 60-120-fold. The rescue with imidlazole was pH-and concentration-dependent. The pH- activity profiles showed nearly bell-shaped curves, with the maximum activity enhancement for H134A at pH 6.0 and that for H252A at pH 7.5. These findings indicated that the prot...
The deoxyribonucleases1 of animal tissues are of two types which are readily distinguishable by the ...
Proteins are known as the workhorses of the cell and fulfill numerous tasks essential for the cell’s...
We previously demonstrated—through the isolation of RNA-cleaving deoxyribozymes by in vitro selec-ti...
Previous structural studies based on the co-crystal of a complex between bovine pancreatic deoxyribo...
One large essential (C173-C209) and one small nonessential ( C101-CI04) disulfide loops occur in bov...
The three-dimensional structure of bovine pancreatic (bp) DNase revealed that its N- and C-termini f...
Bovine pancreatic deoxyribonuclehse I (bpDNase), the first DNase discovered, is the best characteriz...
A mutant of bovine pancreatic DNase I containing two additional residues in a loop next to C173 has ...
Bovine pancreatic ribonuclease A (RNase A) has been widely used as a convenient model for structural...
Bovine pancreatic ribonuclease A (RNase A) has been widely used as a convenient model for structural...
Bovine pancreatic ribonuclease A (RNase A) has been widely used as a convenient model for structural...
The three-dimensional structure of bovine pancreatic deoxyribonuclease I (DNase I) has been determin...
All organisms replicate DNA to be used during cell division and passed on to offspring. In order for...
AbstractBovine pancreatic ribonuclease A (RNase A) has been widely used as a convenient model for st...
DNase is an enzyme belonging to the endonuclease group. It is the most examined enzyme among endonuc...
The deoxyribonucleases1 of animal tissues are of two types which are readily distinguishable by the ...
Proteins are known as the workhorses of the cell and fulfill numerous tasks essential for the cell’s...
We previously demonstrated—through the isolation of RNA-cleaving deoxyribozymes by in vitro selec-ti...
Previous structural studies based on the co-crystal of a complex between bovine pancreatic deoxyribo...
One large essential (C173-C209) and one small nonessential ( C101-CI04) disulfide loops occur in bov...
The three-dimensional structure of bovine pancreatic (bp) DNase revealed that its N- and C-termini f...
Bovine pancreatic deoxyribonuclehse I (bpDNase), the first DNase discovered, is the best characteriz...
A mutant of bovine pancreatic DNase I containing two additional residues in a loop next to C173 has ...
Bovine pancreatic ribonuclease A (RNase A) has been widely used as a convenient model for structural...
Bovine pancreatic ribonuclease A (RNase A) has been widely used as a convenient model for structural...
Bovine pancreatic ribonuclease A (RNase A) has been widely used as a convenient model for structural...
The three-dimensional structure of bovine pancreatic deoxyribonuclease I (DNase I) has been determin...
All organisms replicate DNA to be used during cell division and passed on to offspring. In order for...
AbstractBovine pancreatic ribonuclease A (RNase A) has been widely used as a convenient model for st...
DNase is an enzyme belonging to the endonuclease group. It is the most examined enzyme among endonuc...
The deoxyribonucleases1 of animal tissues are of two types which are readily distinguishable by the ...
Proteins are known as the workhorses of the cell and fulfill numerous tasks essential for the cell’s...
We previously demonstrated—through the isolation of RNA-cleaving deoxyribozymes by in vitro selec-ti...