A Highly sensitive electrochemical immunosensor for zearalenone using screen-printed disposable electrodes.

Zearalenone (ZEN) is an important non-steroidal estrogenic mycotoxin produced by fusarium fungi species. A number of methods based on enzyme-linked immune assays (ELISA) and chromatographic approaches are available for the detection of ZEN. In this research, ZEN conjugated by bovine serum albumin (BSA) was immobilized on the surface of the screen-printed carbon electrode (SPCE) followed by the subsequent addition of anti-zearalenone monoclonal antibody (ZEN-MAb), alkaline phosphatase (ALP)-labeled secondary antibody, and 1-napthyl phosphate (1-NP) as a substrate. This immunosensor had good specificity, selectivity, reproducibility, and sensitivity for ZEN (LOD of 0.25 ng mL-5) when employed for quantification based on differential pulse voltammetry (DPV). This immunosensor yielded good recovery values in the range of 89-97% against real matrices (i.e., beer and wine samples) after spiking. This novel immunosensing principle can be extensively applied for the development of advanced sensing techniques for various analytes.This research was supported by the North Atlantic Treaty Organization Science for Peace and Security (NATO SPS) programme, project NUKR.SFPP 984637, and a grant from the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT, and Future Planning (Grant No: 2016R1E1A1A01940995). It was also carried out with the support of "Cooperative Research Program for Agriculture Science and Technology Development (Grant No: PJ012521032017)" Rural Development Administration, Republic of Korea. This research also acknowledges the support made by the R&D Center for Green Patrol Technologies through the R&D for Global Top Environmental Technologies funded by the Ministry of Environment (MOE)