In brain tumor surgery, recognition of tumor boundaries is key. However, intraoperative assessment of tumor boundaries by the neurosurgeon is difficult. Therefore, there is an urgent need for tools that provide the neurosurgeon with pathological information during the operation. We show that third harmonic generation (THG) microscopy provides label-free, real-time images of histopathological quality; increased cellularity, nuclear pleomorphism, and rarefaction of neuropil in fresh, unstained human brain tissue could be clearly recognized. We further demonstrate THG images taken with a GRIN objective, as a step toward in situ THG microendoscopy of tumor boundaries. THG imaging is thus a promising tool for optical biopsies
Second-harmonic generation (SHG) microscopy has shown great promise for imaging live cells and tissu...
Management of gliomas requires an invasive treatment strategy, including extensive surgical resectio...
We show a portable third and second harmonic generation microscope in the clinic generating 3D real-...
In brain tumor surgery, recognition of tumor boundaries is key. However, intraoperative assessment o...
BACKGROUND Label-free optical techniques such as optical coherence tomography and Raman techniques a...
Distinguishing tumors from normal brain cells is important but challenging in glioma surgery due to ...
Distinguishing tumors from normal brain cells is important but challenging in glioma surgery due to ...
Third harmonic generation (THG) microscopy is a label-free imaging technique that shows great potent...
The ability to visualize neurons inside living brain tissue is a fundamental requirement in neurosci...
Real‐time assessment of excised tissue may help to improve surgical results in breast tumor surgerie...
Abstract The ability to distinguish and grade malignant cells during surgical procedures in a fast, ...
Currently the gold standard for analyzing malignant breast tissue is H&E staining. However this requ...
Motivation: The morphologies contained in 3D third harmonic generation (THG) images of human brain t...
The interaction of cells within their microenvironmental niche is fundamental to cell migration, pos...
Second-harmonic generation (SHG) microscopy has shown great promise for imaging live cells and tissu...
Management of gliomas requires an invasive treatment strategy, including extensive surgical resectio...
We show a portable third and second harmonic generation microscope in the clinic generating 3D real-...
In brain tumor surgery, recognition of tumor boundaries is key. However, intraoperative assessment o...
BACKGROUND Label-free optical techniques such as optical coherence tomography and Raman techniques a...
Distinguishing tumors from normal brain cells is important but challenging in glioma surgery due to ...
Distinguishing tumors from normal brain cells is important but challenging in glioma surgery due to ...
Third harmonic generation (THG) microscopy is a label-free imaging technique that shows great potent...
The ability to visualize neurons inside living brain tissue is a fundamental requirement in neurosci...
Real‐time assessment of excised tissue may help to improve surgical results in breast tumor surgerie...
Abstract The ability to distinguish and grade malignant cells during surgical procedures in a fast, ...
Currently the gold standard for analyzing malignant breast tissue is H&E staining. However this requ...
Motivation: The morphologies contained in 3D third harmonic generation (THG) images of human brain t...
The interaction of cells within their microenvironmental niche is fundamental to cell migration, pos...
Second-harmonic generation (SHG) microscopy has shown great promise for imaging live cells and tissu...
Management of gliomas requires an invasive treatment strategy, including extensive surgical resectio...
We show a portable third and second harmonic generation microscope in the clinic generating 3D real-...