There is a dearth of approaches to experimentally direct cell migration by continuously varying signal input to a single cell, evoking all possible migratory responses and quantitatively monitoring the cellular and molecular response dynamics. Here we used a visual blue opsin to recruit the endogenous G-protein network that mediates immune cell migration. Specific optical inputs to this optical trigger of signaling helped steer migration in all possible directions with precision. Spectrally selective imaging was used to monitor cell-wide phosphatidylinositol (3,4,5)-triphosphate (PIP3), cytoskeletal, and cellular dynamics. A switch-like PIP3 increase at the cell front and a decrease at the back were identified, underlying the decisive migra...
Regulation of G-protein signaling occurs spatially between microdomains of cellular membranes, and t...
Cells must migrate for neurons to make connections with distant partners, immune cells to find their...
G-protein-coupled receptors (GPCRs) stimulate signaling networks that control a variety of critical ...
ABSTRACT Cells sense gradients of extracellular cues and generate polarized responses such as cell m...
Background Tight spatio-temporal signaling of cytoskeletal and adhesion dynamics is required for lo...
AbstractIt has long been appreciated that spatiotemporal dynamics of cell migration are under the co...
BACKGROUND Tight spatio-temporal signaling of cytoskeletal and adhesion dynamics is required for ...
In order to track cell migration, the project used a transgenic mouse system which ubiquitously expr...
Tight spatio-temporal signaling of cytoskeletal and adhesion dynamics is required for localized memb...
Numerous molecular components have been identified that regulate the directed migration of eukaryoti...
Cell motility plays an integral role in most biological processes. One principle of motility is the ...
Numerous molecular components have been identified that regulate the directed migration of eukaryoti...
Active migration in both healthy and malignant cells requires the integration of information derived...
The accumulation of antigen specific T lymphocytes at sites of antigenic challenge is an important p...
The accumulation of antigen specific T lymphocytes at sites of antigenic challenge is an important p...
Regulation of G-protein signaling occurs spatially between microdomains of cellular membranes, and t...
Cells must migrate for neurons to make connections with distant partners, immune cells to find their...
G-protein-coupled receptors (GPCRs) stimulate signaling networks that control a variety of critical ...
ABSTRACT Cells sense gradients of extracellular cues and generate polarized responses such as cell m...
Background Tight spatio-temporal signaling of cytoskeletal and adhesion dynamics is required for lo...
AbstractIt has long been appreciated that spatiotemporal dynamics of cell migration are under the co...
BACKGROUND Tight spatio-temporal signaling of cytoskeletal and adhesion dynamics is required for ...
In order to track cell migration, the project used a transgenic mouse system which ubiquitously expr...
Tight spatio-temporal signaling of cytoskeletal and adhesion dynamics is required for localized memb...
Numerous molecular components have been identified that regulate the directed migration of eukaryoti...
Cell motility plays an integral role in most biological processes. One principle of motility is the ...
Numerous molecular components have been identified that regulate the directed migration of eukaryoti...
Active migration in both healthy and malignant cells requires the integration of information derived...
The accumulation of antigen specific T lymphocytes at sites of antigenic challenge is an important p...
The accumulation of antigen specific T lymphocytes at sites of antigenic challenge is an important p...
Regulation of G-protein signaling occurs spatially between microdomains of cellular membranes, and t...
Cells must migrate for neurons to make connections with distant partners, immune cells to find their...
G-protein-coupled receptors (GPCRs) stimulate signaling networks that control a variety of critical ...