Green Synthesis of Copper Oxide Nanoparticles Using Aloe vera Leaf Extract and Its Antibacterial Activity Against Fish Bacterial Pathogens

The present study reports biologically oriented process for green synthesis of CuO nanoparticles by using ecofriendly and non-toxic Aloe vera leaf extract. Powder X-ray diffraction and transmission electron microscope analysis revealed that synthesized CuO nanoparticles are in monoclinic phase with average particle size of 20 nm. The antibacterial activity of green synthesized CuO nanoparticles was tested against three bacterial fish pathogens "viz" Aeromonas hydrophila, Pseudomonas fluorescens and Flavobacterium branchiophilum, which are responsible for causing severe infectious diseases in fishes. CuO NPs exhibits enhanced antibacterial activity against all the fish pathogens even at lower concentrations, i.e. above 20 mu g/mL