Micropropagation, Establishment Of Cell Suspension Cultures And Evaluation Of Antioxidant Activities Of Gynura Procumbens

Aseptic bud explants of Gynura procumbens, obtained from the axillary buds of field grown mature plants, could be established via surface-sterilization with 20 % Clorox® for 20 minutes. The formation of multiple shoots could be induced on MS medium supplemented with 2.0 mg/L BA with an average of 14.7 ± 0.7 shoots produced from each bud explant after four weeks of culture. When the concentration of BA supplemented into the MS medium was reduced to 1.5 mg/L BA, reasonably high number of shoots (13.9 ± 1.3 shoots per explant) can still be produced with less production of abnormal shoots