Clinicopathologic Findings in Cows Inoculated with an Inactivated Bovine Salmonella Vaccine Containing a Lipopolysaccharide Neutralizer

サルモネラ・ティフィムリウム及び同ダブリンの不活化菌体抗原を主成分とし，エンドトキシン中和剤であるポリミキシンB硫酸塩を含む牛サルモネラ症不活化ワクチンを，健常なホルスタイン種育成牛8頭に接種した際の生体の反応を，臨床病理学的に観察した．ワクチン接種後に食欲の低下や一般状態の悪化は認められなかった．注射部位に一過性の腫脹及び硬結が認められた．血液検査所見では，ワクチン接種後24時間以内に，急性炎症の指標となるヘマトクリット値，血清総蛋白質濃度，アスパラギン酸アミノトランスフェラーゼ活性値，クレアチンキナーゼ活性値及びシアル酸濃度の有意な上昇が認められた．一方，ワクチン接種後にエンドトキシンショックや過敏症反応を疑わせる臨床症状は認められなかった．免疫学的な有効性が認められて市販されている本ワクチンは，接種部位の局所的な急性炎症を起こすが，重篤な副反応を引き起こす可能性は低いと考えられた．Eight healthy Holstein dairy cows were inoculated with an inactivated bovine salmonellosis vaccine that was composed primarily of inactivated somatic antigens of Salmonella typhimurium and Salmonella enterica serovar Dublin and contained polymixin B sulfate, an endotoxin neutralizer. Clinicopathologic responses were observed. After vaccination, neither a decline in appetite nor deterioration in their general condition was noted. Swelling and induration were recognized at the inoculation site. A hematological examination revealed significant post-vaccination changes in the hematocrit, albumin-to-globulin ratio, total protein concentration, aspartate aminotransferase activity, creatine kinase activity and sialic acid concentration in the serum, all of which are markers of acute inflammation. However, no clinical manifestations suggestive of endotoxic shock or anaphylactoid reaction were noted after the vaccination. Although this vaccine causes local acute inflammation at the inoculation site, the likelihood of severe side-effects is low. Therefore, the vaccine can be used effectively in clinical settings