Biochemical characteristics peroxidase from pineapple (Ananas comosus (L.) Merrill): cultivar IAC Gomo-de-mel and clone IAC-1

No presente trabalho foram estudadas as características bioquímicas das peroxidases de novos abacaxis, cultivar IAC Gomo-demel e clone IAC-1. As peroxidases dos sucos destes abacaxis apresentaram atividade ótima entre 45ºC e 50°C e entre 50°C e 55°C, respectivamente. Estas peroxidases apresentaram atividade ótima em pH 4,5 e mostraram-se estáveis na faixa de pH 4,0 a 9,0, retendo, após 24 horas de incubação a 50ºC, mais de 80% da atividade inicial. Foi observada regeneração parcial da atividade da peroxidase, após tratamento a 75ºC por 10 minutos. As peroxidases dos sucos dos abacaxis estudados foram inativadas, após tratamento a 90ºC por 2 minutos.In the present work, the biochemical characteristics of peroxidase enzymes from new pineapples, cultivar IAC Gomo-de-mel and clone IAC-1, were studied. The peroxidases obtained from the juice of this pineapples presented optimum activities from 45 ºC to 50 ºC and 50 ºC to 55 ºC, respectively. These peroxidases showed maximum activity at pH 4.5 and showed stability at pH 4.0 to 9.0, retaining after 24 hours of incubation at 50 ºC more than 80% of initial activity. Partial regeneration of peroxidase activity was observed after treatment at 75 ºC for 10 minutes. The peroxidases from pineapple juices studied were inactivated after treatment at 90 ºC for 2 minutes