Microbial pathogens such as bacillus Calmette-Guérin (BCG) induce the activation of macrophages. Activated macrophages can be characterized by the increased production of reactive oxygen and nitrogen metabolites, generated via NADPH oxidase and inducible nitric oxide synthase, respectively, and by the increased expression of major histocompatibility complex class II molecules (MHC II). Multiple microassays have been developed to measure these parameters. Usually each assay requires 2-5 x 10(5) cells per well. In some experimental conditions the number of cells is the limiting factor for the phenotypic characterization of macrophages. Here we describe a method whereby this limitation can be circumvented. Using a single 96-well microassay and...
Summary: Here, we present optimized approaches to identify the efficiency of cancer cell phagocytosi...
International audienceMacrophages are plastic immune cells that can adopt several activation states....
Macrophages from C3H/HeN mice treated in vivo with Mycobacterium bovis, strain BCG or in vitro with ...
Macrophages are mononuclear phagocytes derived from haematopoietic progenitors that are widely distr...
The purpose of this experiment is to develop comparative methodology for analyzing RAW 264.7 murine ...
The chapter, "Generation and characterization of murine alternatively activated macrophages" was wri...
The tumoricidal activity of activated macrophages has been attributed largely to the release of tumo...
In the mouse, the trait of innate resistance or susceptibility to infection with several species of ...
Nitric oxide (NO*) is a critical component of mammalian host defense that is produced in macrophages...
Direct comparison of Bone Marrow Derived Macrophages (BMDM) to the murine derived RAW 264.7 cell lin...
<p>Thioglycolate-elicited macrophages were harvested from the peritoneal cavity of C57BL/6 WT and Ph...
Macrophages are derived from hematopoietic progenitor cells throughout the body, are central to infl...
<p>Bone marrow-derived macrophages from ApoE<sup>−/−</sup> and γ<sup>−/−</sup>ApoE<sup>−/−</sup> mic...
Macrophages are first responders , innate immune system cells which quickly arrive to a site of inf...
Macrophage activation refers to the enhanced functionality of macrophages in response to endogenous ...
Summary: Here, we present optimized approaches to identify the efficiency of cancer cell phagocytosi...
International audienceMacrophages are plastic immune cells that can adopt several activation states....
Macrophages from C3H/HeN mice treated in vivo with Mycobacterium bovis, strain BCG or in vitro with ...
Macrophages are mononuclear phagocytes derived from haematopoietic progenitors that are widely distr...
The purpose of this experiment is to develop comparative methodology for analyzing RAW 264.7 murine ...
The chapter, "Generation and characterization of murine alternatively activated macrophages" was wri...
The tumoricidal activity of activated macrophages has been attributed largely to the release of tumo...
In the mouse, the trait of innate resistance or susceptibility to infection with several species of ...
Nitric oxide (NO*) is a critical component of mammalian host defense that is produced in macrophages...
Direct comparison of Bone Marrow Derived Macrophages (BMDM) to the murine derived RAW 264.7 cell lin...
<p>Thioglycolate-elicited macrophages were harvested from the peritoneal cavity of C57BL/6 WT and Ph...
Macrophages are derived from hematopoietic progenitor cells throughout the body, are central to infl...
<p>Bone marrow-derived macrophages from ApoE<sup>−/−</sup> and γ<sup>−/−</sup>ApoE<sup>−/−</sup> mic...
Macrophages are first responders , innate immune system cells which quickly arrive to a site of inf...
Macrophage activation refers to the enhanced functionality of macrophages in response to endogenous ...
Summary: Here, we present optimized approaches to identify the efficiency of cancer cell phagocytosi...
International audienceMacrophages are plastic immune cells that can adopt several activation states....
Macrophages from C3H/HeN mice treated in vivo with Mycobacterium bovis, strain BCG or in vitro with ...